Clinicians need reliable methods to determine the identity of bacteria that have been isolated from patients. Staining methods, media-based tests, and biochemical tests are all used to determine the genus and species of unknown bacteria.
Objectives:
- Identify an unknown microorganism from a given broth culture using various selective media.
- Perform an isolation streak of an unknown bacterium, and describe the color, shape, and margin of the resulting colonies.
- Explain the steps used in a Gram stain and describe the cell wall structure, morphology, and arrangement of an unknown bacteria.
- List the selective and differential components present in any media used, and explain the function of each selective and/or differential component.
- Explain the biochemical test, identify the enzyme for which the biochemical test is assaying, and interpret the results.
- Describe the rapid membrane test used on an unknown bacterium, and interpret the results.
- Determine the genus and species of an unknown bacterium using the dichotomous key.
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